The present invention discloses a positioning guide for being disposed on a body, and includes a first body having a first setting portion; a second body adjacent to the first body, having a The second setting portion has a geometric central axis coaxial with the axis of the second body. The positioning guide provided by the present invention can effectively achieve the effect of assisting the operator to improve the accuracy of the puncture and reduce the incidence of complications.本創作係揭露一種定位導引器,其係用於設於一架體上,而包含有一第一本體,具有一第一設置部;一第二本體,鄰設於該第一本體,具有一第二設置部,其幾何中心軸與該第二本體之軸為同軸。藉由本創作提供之定位導引器,其係能夠有效達到輔助操作者能提高穿刺之精確度及降低併發症發生率之功效。