The proposed improved formulation for sustained release of plant growth hormone and process of preparation thereof is prepared by intercalation of 6-BAP into the MMT interlayer by electrostatic interaction forming 6-BAP-MMT hybrid which is further entrapped in biopolymer AL to form 6-BAP-MMT-AL beads which provide sustained release of 6-BAP in a prolonged and sustained manner preventing 6-BAP oxidation. The proposed formulation releases a plant growth hormone 6-BAP from 6-BAP-MMT-AL beads in presence of water having pH in range of 7.2-7.4 in controlled release manner for a period of 170hours (Fig.5).