Pao-Chi Liao,Ying-Hwa Chang,Kuo-Hsun Chiu,Yu-Shun Wu,Shu-Hui Lee
申请号：
US13338020
公开号：
US08912155B2
申请日:
2011.12.27
申请国别(地区):
US
年份:
2014
代理人:
摘要：
A method for analyzing secretome, a biomarker for lung cancer metastasis, and a siRNA compound for inhibiting lung cancer metastasis are disclosed. The method for analyzing secretome of the present invention comprises the following steps: (A) collecting proteome secreted from a cell (B) providing a purification gel, wherein the purification gel comprises a low-density layer, and a high-density layer, and the low-density layer is stacked on the high-density layer (C) adding the proteome on the low-density layer, and separating the proteome through the low-density layer and the high-density layer of the purification gel (D) collecting the separated proteome on the interface between the low-density layer and high-density layer, and tagging the separated proteome with a reagent after digestion and (E) analyzing the separated proteome tagged with the reagent, and comparing an analysis result with a proteomic database.
