Means for the preparation of neural stem cells, characterized in that it comprises: a basic medium for the culture of a stem cell, and an inhibitor consisting of a GSK inhibitor, a MEK inhibitor, a TGF-b inhibitor, an inhibitor of ROCK and a BMP inhibitor.Medio para la preparación de células madre neurales, caracterizado por que comprende: un medio básico para el cultivo de una célula madre, y un inhibidor que consiste en un inhibidor de GSK, un inhibidor de MEK, un inhibidor de TGF-b, un inhibidor de ROCK y un inhibidor de BMP.