FIELD: biotechnology.SUBSTANCE: method provides a storage mode of cultivation, namely the actively dividing culture of Cylindrotheca closterium diatom, taken at the linear stage of growth, with an initial density of 0.1–0.2 g of dry matter per 1 liter of culture, are cultured for 10-12 days in photobioreactors in the form of cultivators of plane-parallel type with a thickness of the illuminated layer of 5 cm, on a modified nutrient medium prepared on the basis of sterilized sea water, g⋅l, with the following composition: NaNO- 7.50, NaSiO×9HO - 3.00, NaHPO×2HO - 0.50, NaEDTA - 0.872, FeSO×7HO - 0.63, NaMoO×HO - 0.063, CuSO×5HO - 0.1, ZnSO×7HO - 0.22, CoCl×6HO - 0.1, MnCl×4HO - 0.18.EFFECT: method makes it possible to increase the content of fucoxanthin in the biomass of Cylindrotheca closterium diatom.1 cl, 3 dwg, 1 tbl, 2 ex