ТЕ ГАВЕРНМЕНТ ОФ ТЕ ЮНАЙТЕД СТЕЙТС ОФ АМЕРИКА ЭЗ РЕПРИЗЕНТЕД БАЙ ТЕ СЕКРЕТАРИ ОФ ТЕ ДЕПАРТМЕНТ ХЕЛТ ЭНД ХЬЮМАН СЕРВИСЕЗ
发明人:
ПАСТАН Ира Х.,БИРС Ричард,ОНДА Масанори
申请号：
RU2012114005
公开号：
RU2012114005A
申请日:
2010.09.10
申请国别(地区):
RU
年份:
2013
代理人:
摘要：
1. Isolated exotoxin A Pseudomonas ( «PE"), wherein in said PE deleted residues 1-273 and 285-394 produced and substituted for alanine, glycine, or serine in place of amino acid residues D406, R432, R467, R490, R513, E548, K590 and Q592, the corresponding amino acid residue sequence of SEQ ID NO: 1.2. PE according to claim 1, wherein said PE has a further substitution with alanine, glycine or serine, at least one amino acid residue corresponding to amino acid residues sequence of SEQ ID NO: 1, which is selected from the group consisting of D403, R412, R427, E431, R458, D461, R505, E522, R538, R551, R576 and L597.3. PE according to claim 1, wherein said PE has an amino acid sequence of SEQ ID NO: 2.4. PE according to claim 1 or 3, wherein said PE has an amino acid sequence of SEQ ID NO: 3.5. A chimeric molecule comprising (a) a guide portion, conjugated or fused to (b) exotoxin A Pseudomonas ( «PE"), wherein said PE deleted residues 1-273 and 285-394 produced and substituted for an alanine, glycine or serine at site residues D406, R432, R467, R490, R513, E548, K590 and Q592, corresponding to amino acid residues sequence of SEQ ID NO: 1.6. The chimeric molecule of claim 5, wherein said PE has a further substitution with alanine, glycine or serine, at least one amino acid residue corresponding to amino acid residues sequence of SEQ ID NO: 1, which is selected from the group consisting of D403, R412, R427, E431, R458, D461, R505, E522, R538, R551, R576 and L597.7. The chimeric molecule of claim 5, wherein said PE has an amino acid sequence of SEQ ID NO: 2.8. The chimeric molecule of claim 5, wherein said PE has an amino acid sequence of SEQ ID NO: 3.9. The chimeric molecule of claim 5, wherein said guide portion is a1. Изолированный экзотоксин A Pseudomonas («РЕ»), где в указанном РЕ удалены остатки 1-273 и 285-394 и произведены замены на аланин, глицин или серин на месте аминокислотных остатков D406, R432, R467, R490, R513, Е548, К590 и Q592, соответствующих аминокисло
