JAMES VAN ALSTINE,JEMIL SHANAGAR,ROLF HJORTH,KAROL LACKI
申请号：
IN4348/DELNP/2011
公开号：
IN2011DN04348A
申请日:
2011.06.09
申请国别(地区):
IN
年份:
2012
代理人:
摘要：
The present invention relates to methods of isolating biomolecules. More particularly, the invention relates to methods for isolating antibodies (mAbs) and related proteins including antibody fragments (Fabs) under conditions where they are positive and relatively hydrophobic and will react with negatively charged polymer to form polymer-protein complexes which precipitate. The isolation can be accomplished using inexpensive and biocompatible negatively charged polymers such as polyacrylic acid or carboxymethyldextran polymers of various molecular weights as precipitant. It occurs at relatively high concentrations of polymer (e.g. 10%) and high salt concentration (>50 mM) and conductivity (e.g. >10 mS/cm) over wide range of pH.
