METHOD OF CULTIVATION OF HUMAN MESENCHYMAL STEM CELLS, PARTICULARLY FOR THE TREATMENT OF NON-HEALING FRACTURES, AND BIOREACTOR FOR CARRYING OUT THIS CULTIVATION METHOD
UNIVERZITA KARLOVA V PRAZE;USTAV HEMATOLOGIE A KREVNI TRANSFUZE;USTAV MAKROMOLEKULARNI CHEMIE AV CR, V.V.I.;PYTLIK, ROBERT;HOFMAN, PETR;TRC, TOMAS;STEHLIK, DAVID;SOUKUP, TOMAS;KOBYLKA, PETR;KLENER, PAVEL;RYPACEK, FRANTISEK;MULINKOVA, KATARINA
发明人:
PYTLIK, ROBERT,HOFMAN, PETR,TRC, TOMAS,STEHLIK, DAVID,SOUKUP, TOMAS,KOBYLKA, PETR,KLENER, PAVEL,RYPACEK, FRANTISEK,MULINKOVA, KATARINA
申请号：
WO2007CZ00005
公开号：
WO2007085210(A3)
申请日:
2007.01.23
申请国别(地区):
世界知识产权组织国际局
年份:
2008
代理人:
摘要：
The invention relates to a method of cultivation of mesenchymal stem cells, wherein after aseptic separation of mononuclear cells from the marrow blood, said cells are seeded in low density into sterile plastic cultivation vessels and cultivated for approximately one to three weeks in CellGroTM Hematopoietic Stem Cell Medium, certified for the clinical use, with an addition of 10% human serum and supplements, wherein the supplements are added at least once in the course of the cultivation, without removal of hematopoietic cells and without medium exchange during the cultivation procedure, without any interference with the closed cultivation system, under the standard conditions for the cultivation of tissue cultures. For the cultivation of the mesenchymal stem cells in the closed cultivation system for the clinical use in the field of orthopaedic surgery, a simple bioreactor is proposed. The bioreactor consists of a cassette system containing cultivation vessels with filters for securing the sterile exchange of gas and with aseptic inlets for seeding and harvesting the cells and adding the supplements, and a carrier.
