The present invention provides a composition for inhibiting the activity of an urease, including at least one acid component, at least one base component and an urease activity inhibitor, wherein the at least one acid component is one of an organic acid, a phosphoric acid and a combination thereof, the at least one base component is one of an organic base, a phosphate and a combination thereof, the at least one acid component and the at least one base component conjugate with each other to form a buffer formulation, and the urease activity inhibitor is one of an ascorbic acid, its salt and a combination thereof, wherein the buffer solution is used for maintaining the activity of the urease activity inhibitor.本發明為一種抑制尿素酶之一活性之組合物，包括至少一酸成分，其中該至少一酸成分係一有機酸、一磷酸及其組合其中之一；至少一鹼成分，其中該至少一鹼成分係一有機鹼、一磷酸鹼及其組合其中之一、該至少一酸成分及該至少一鹼成分具一共軛關係、且該至少一酸成分及該至少一鹼成分形成一緩衝配方；以及一尿素酶活性抑制劑，為一抗壞血酸、其鹽類及其組合其中之一，其中該緩衝配方用以穩定該尿素酶活性抑制劑的活性。
