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a way to improve the ability to resist plant dna containing viruses, plants
专利权人:
发明人:
Каиксиа,Ксианг,Хуавей
申请号:
EA201791991
公开号:
EA201791991A1
申请日:
2016.03.14
申请国别(地区):
EA
年份:
2018
代理人:
摘要:
the invention discloses a way to improve the ability to resist \u0432\u043d\u0435\u0434\u0440\u0435\u043d\u043d\u043e\u043c\u0443 dna containing virus of plants.the invention provides a method of obtaining plants with enhanced ability to dna containing virus that includes, in particular, as follows e stages: 1) the selection of sequences.the 5 - Nx - NGG - 3 or 5 - CCN - Nx - 3 from genome dna sequences containing the virus as the target sequence design of dna sequences.back of complementary target sequence (2) the embedding sequence dna vector for the expression of \u043d\u0443\u043a\u043b\u0435\u0430\u0437\u044b CRISPR \/ Cas9 to obtain with a vectorcapable of \u0442\u0440\u0430\u043d\u0441\u043a\u0440\u0438\u0431\u0438\u0440\u043e\u0432\u0430\u0442\u044c guided rna and protein Cas9 \u044d\u043a\u0441\u043f\u0440\u0435\u0441\u0441\u0438\u0440\u043e\u0432\u0430\u0442\u044c cr\u0440\u043d\u043a in directing rna contains a fragment of rna.\u0442\u0440\u0430\u043d\u0441\u043a\u0440\u0438\u0431\u0438\u0440\u043e\u0432\u0430\u043d\u043d\u044b\u0439 with dna fragment (3) the introduction of a plant with the recipient to produce plants with enhanced ability to \u043f\u0440\u043e\u0442\u0438\u0432\u043e\u0434\u0435\u0439\u0441\u0442\u0432\u043e\u0432\u0430\u0442 it is a dna virus.method in this invention is based only on the genome sequence of the virus, and does not require knowledge of the specific functions of viral genes.therefore, the method can be widely applied to counter various dna containing viruses.the genome of which contains \u0434\u0432\u0443\u0445\u0446\u0435\u043f\u043e\u0447\u0435\u0447\u043d\u0443\u044e dna or single strand dna with \u0434\u0432\u0443\u0445\u0446\u0435\u043f\u043e\u0447\u0435\u0447\u043d\u043e\u0439 as mediator.В изобретении раскрывается способ улучшения способности противодействовать внедренному ДНК-содержащему вирусу растения. Изобретение обеспечивает способ получения растения с улучшенной способностью противодействовать ДНК-содержащему
来源网站:
中国工程科技知识中心
来源网址:
http://www.ckcest.cn/home/

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