THE TRUSTEES OF THE UNIVERSITY OF PENNSYLVANIA;UNIVERSITY OF FLORIDA RESEARCH FOUNDATION, INC.
发明人:
Roland W. Herzog,Henry Daniell
申请号：
US16787778
公开号：
US20200270313A1
申请日:
2020.02.11
申请国别(地区):
US
年份:
2020
代理人:
摘要：
Protein replacement therapy for patients with haemophilia or other inherited protein deficiencies is often complicated by pathogenic antibody responses, including antibodies that neutralize the therapeutic protein or that predispose to potentially life-threatening anaphylactic reactions by formation of IgE. Using murine and canine haemophilia as a model, we have developed a prophylactic protocol. Against such responses that is non-invasive and does not include immune suppression mor genetic manipulation of the patient's cells. Oral delivery of a coagulation factor expressed in chloroplasts, bioencapsulated in plant cells, effectively blocked formation of inhibitory antibodies in protein replacement therapy. Inhibitor titers were mostly undetectable and up to 100-fold lower in treated subjects when compared to controls. Moreover, this treatment eliminated fatal anaphylactic reactions that occurred after four to six exposures to intravenous coagulation factor protein. Finally, the method can effectively be used to reverse or reduce undesirable pre-existing inhibitor titers.
