METHOD FOR PREPARING CELL AGGREGATE DERIVED FROM CANCER TISSUES, AND ANTICANCER DRUG SCREENING METHOD USING CELL AGGREGATE DERIVED FROM CANCER TISSUES, QUANTITATIVE ANALYSIS OF ANTICANCER AGENT, OR RADIOSENSITIVITY TEST OF CANCER TISSUES
PROBLEM TO BE SOLVED: To provide a method for preparing a cell aggregate derived from cancer tissues which can prepare a large amount of assemblage of cell aggregate derived from cancer tissues which is homogeneous and equal in cell numbers.SOLUTION: The invention provides a method comprising culturing to proliferate cells in the condition of embedding a cell aggregate obtained from a cancer tissue or a cell aggregate obtained by culturing it into a substrate for cell support, converting the cell aggregate after culturing into a single cell, and culturing the obtained cell to form a cell aggregate in an incubator which has a rugged structure whose interval between convex parts is smaller than the size of the cell.COPYRIGHT: (C)2015,JPO&INPIT【課題】均質で細胞数の揃った癌組織由来細胞凝集塊の集合を大量に調製可能な癌組織由来細胞凝集塊の調製方法を提供する。【解決手段】 癌組織から得た細胞塊又はそれを培養して得られた細胞凝集塊を細胞支持用基質に包埋した状態で培養して細胞を増殖させる工程と、培養後の細胞塊を単一細胞化する工程と、得られた細胞を、凸部間の間隔が細胞の大きさより小さな凹凸構造を有する培養器中で培養して、細胞凝集塊を形成する工程とを含む方法とする。【選択図】なし
