CDNA corresponding to the antigenome of nonsegmented negative strand RNA viruses, and process for the production of such viruses encoding additional antigenically active proteins
Frank Radecke;Pius Spielhofer;Karin Kalin;Henriette Schneider;Martin A. Billeter
发明人:
Martin A. Billeter,Pius Spielhofer,Karin Kalin,Frank Radecke,Henriette Schneider
申请号：
US13175307
公开号：
US08158416B2
申请日:
2011.07.01
申请国别(地区):
US
年份:
2012
代理人:
摘要：
The present invention relates, in general, to a methodology for the generation of nonsegmented negative-strand RNA viruses (Pringle, 1991) from cloned deoxyribonucleic acid (cDNA). Such rescued viruses are suitable for use as vaccines, or alternatively, as plasmids in somatic gene therapy applications. The invention also relates to cDNA molecules suitable as tools in this methodology and to helper cell lines allowing the direct rescue of such viruses. Measles virus (MV) is used as a mode for other representatives of the Mononegavirales, in particular the family Paramyxoviridae.
