The device (1) for collection of animal semen comprises a rigid (2) which is adaptable to an artificial vagina (V), and to which filtration means (3) and a collection receptacle (4) are fixed, the filtration means (3) being positioned inside the collection receptacle (4). The filtration means form a filtration pouch, preferably conical, that make it possible to hold back the “tapioca” and to allow the liquid fractions of the semen, including the “rich fraction” containing the spermatozoa, to pass into the collection receptacle (4).