The device (1) for collection of animal semen comprises a rigid frame (2)which is adaptable to an artificial vagina (V), and to which filtration means(3) and a collection receptacle (4) are fixed, the filtration means (3) beingpositioned inside the collection receptacle (4). The filtration means form afiltration pouch, preferably conical, that make it possible to hold back the"tapioca" and to allow the liquid fractions of the semen, including the "richfraction" containing the spermatozoa, to pass into the collection receptacle(4).