SILVERMAN JOSHUA,WANG CHIA-WEI,SPINK BENJAMIN,CLELAND JEFFREY L,STEMMER WILLEM P,SCHELLENBERGER VOLKER,GEETHING NATHAN
申请号：
NZ59678710
公开号：
NZ596787A
申请日:
2010.06.08
申请国别(地区):
NZ
年份:
2014
代理人:
摘要：
596787 Disclosed is a fusion protein, comprising a growth hormone (GH) sequence selected from human growth hormone of SEQ ID NO: 1 and an agonist thereof that is at least 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, or 99% identical to the amino acid sequence FPTIPLSRLFDNAMLRAHRLHQLAFDTYQEFEEAYIPKEQKYSFLQNPQTSLCFSESIPTPSNREETQQKSNLELLRISLLLIQSWLEPVQFLRSVFANSLVYGASDSNVYDLLKDLEEGIQTLMGRLEDGSPRTGQIFKQTYSKFDTNSHNDDALLKNYGLLYCFRKDMDKVETFLRIVQCRSVEGSCGF, wherein said GH is linked to an extended recombinant polypeptide (XTEN) of at least about 200 amino acid residues, wherein the XTEN is characterized in that: (a) the XTEN sequence comprises at least about 200 contiguous amino acids that exhibits at least 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, or 99% sequence identity to a comparable length of an amino acid sequence selected from sequences such as MAEPAGSPTSTEEGTPGSGTASSSPGSSTPSGATGSPGASPGTSSTGS and MAEPAGSPTSTEEGASPGTSSTGSPGSSTPSGATGSPGSSTPSGATGS (b) the XTEN sequence lacks a predicted T-cell epitope when analyzed by TEPITOPE algorithm, wherein the TEPITOPE algorithm prediction for epitopes within the XTEN sequence is based on a score of &ndash9 or greater (c) it has a subsequence score of less than 10 and (d) the sum of glycine (G), alanine (A), serine (S), threonine (T), glutamate (E) and proline (P) residues constitutes more than about 90% of the total amino acid residues of the XTEN.
