A method includes steps of: measuring a variation in joint angle of a knee of a cyclist to obtain joint angle data; sensing a torque applied on a crank of a cycle or pedaling force applied to a pedal of the cycle to obtain force data; measuring an angular position of the crank to obtain crank angle data; measuring an electrical potential variation of a measured muscle of the cyclist to generate a measured EMG signal; estimating activation data and joint moment data based on aforementioned data and characteristics data; converting the activation data into estimated EMG signals based on the measured EMG signal and the activation data; and computing a coactivation parameter based on the aforementioned EMG signals.