The present invention discloses a composition of glycine tomentella hayata for use in fiber, fabric or cloth, wherein a plant of glycine tomentella hayata is processed into a variety of micro forms or extracts for being mixed or adsorbed onto the fiber, fabric or cloth. Through antibacterial effect of the ingredient of glycine tomentella hayata, the finished fiber products can have effects of antibacterial, deodorant, antiseptic, antifungal, deodorizing, health care and the like when they are used in underwear, towels, socks or protectors. The preparation method mainly comprising: uniformly mixing the fine powder of glycine tomentella hayata into drawn-work, namely, using a pulverizer to grind the plant of glycine tomentella hayata directly into fine powder, evenly mixing the fine powder of glycine tomentella hayata into a textile base material, followed by drawing into fiber yarn. The ratio of the glycine tomentella hayata fine powder is 2% to 4%.本發明「一條根成份於纖維、織物或布料之用途」,係將一條根植物加工成各種微小型態或萃取物後,混入或吸附在纖維、織物或布料中,藉由一條根成份之抗菌作用,使該纖維成品應用於衣褲、毛巾、襪子或護具後,具有抑菌、防臭、殺菌、防霉、消臭與保健醫療等功效。