Method and composition is provided in the subject for needing it in corneal dystrophy. In one aspect, the method comprising the steps of: obtaining corneal dystrophy target nucleic acid that a large amount of stem cells include nucleic acid mutation from multiple stem cells of object and manipulation nucleic acid mutation one or more stem cell to correct nucleic acid mutation, one or more manipulation stem cells are consequently formed. Processed stem cell separation, is then transplanted to object. In some embodiments, the operation using CRISPR systems of nucleic acid mutation.
