De novo papilla comprising a cellular aggregate of mammalian dermal hair papilla (DPF) fibroblasts, the cellular aggregate exhibiting the shape and size of a physiological dermal papilla (DP) of a hair follicle after its isolation and after its coating with extracellular matrix proteins, preferably collagen IV, fibronectin and / or laminin, where de novo papilla is produced by a method comprising the steps of: (a) providing at least one dermal papilla (DP) of at least one hair follicle of mammal, (b) isolate dermal papillary fibroblasts (DPF) from the DP by mechanically fixing said DP on the surface of a cell culture vessel, according to which the base sheet is perforated to allow said DPF to migrate , (c) extending the isolated DPFs in monolayer culture without collagen coating, in which said DPFs are passed at least once, (d) condensing the extended DPFs into a cell aggregate that they have the size and shape of the physiological DP, in which said DPFs differ in non-adhesive culture vessels in a concentration of cells per surface of the container from 1,000 to 100,000 DPF / cm2, and (e) coating the de novo papilla with extracellular matrix proteins, preferably collagen IV, fibronectin and / or laminin.Papila de novo que comprende un agregado celular de fibroblastos de papila pilosa dérmica (DPF) de mamífero, exhibiendo el agregado celular la forma y el tamaño de una papila dérmica (DP) fisiológica de un folículo piloso tras su aislamiento y después de su recubrimiento con proteínas de matriz extracelular, preferiblemente colágeno IV, fibronectina y/o laminina, donde la papila de novo se produce mediante un método que comprende las etapas de: (a) proporcionar al menos una papila dérmica (DP) de al menos un folículo piloso de mamífero, (b) aislar fibroblastos de papila pilosa dérmica (las DPF) de la DP por fijación de manera mecánica de dicha DP en la superficie de un recipiente de cultivo celular, según lo cual la lámina basal se perfora para permitir que migren di
