The present invention have objects to provide an option of non-reducing saccharide by providing a novel non-reducing saccharide composed of glucose as constituents and to provide a novel enzyme forming the non-reducing saccharide, a method and process for producing the same, a DNA encoding the enzyme, a recombinant DNA and transformant comprising the DNA, a composition comprising the non-reducing saccharide, and uses thereof. The present invention solves the above objects by providing an isocyclomaltooligosaccharide(s) having a structure represented by General Formula 1, a novel isocyclomaltooligosaccharide-forming enzyme, a method and process for producing the same, a DNA encoding the enzyme, a recombinant DNA and transformant comprising the DNA, a composition comprising the isocyclomaltooligosaccharide (s) or a saccharide composition comprising the same, and uses thereof. €ƒ€ƒ€ƒ€ƒ€ƒ€ƒ€ƒ€ƒ General Formula 1 Cyclo {†’6)-[±-D-Glc p -(1†’4)] n -±-D-Glc p -(1†’} (In General Formula 1, "n" means a number of 4 or 5)
