AVIAN INFLUENZA VIRUS ANTIGEN, AND BOOSTER IMMUNIZATION METHOD FOR AVIAN INFLUENZA VACCINE IN COMBINATION WITH MUCOSAL ADJUVANT WHICH IS EFFECTIVE THROUGH ORAL ADMINISTRATION
PROBLEM TO BE SOLVED: To provide an immunization material that enables longer maintenance of immunity induced by an initial immunization with an oil vaccine, at a high level, and that comprises an avian influenza virus antigen and a mucosal adjuvant effective for oral administration.SOLUTION: When ELISA antibody titer dropped in chickens intramuscularly inoculated with an oil vaccine, recombinant HA and NP antigens prepared using gene recombinant technology were orally administered in combination with synthetic oligo CpG as a mucosal adjuvant to the chickens. Even when the immune response induced with the oil vaccine was impaired, the antibody titer was re-induced by orally administering the recombinant HA and NP antigens, and synthetic CpG-ODN in combination.【課題】オイルワクチンによる初回免疫で付与された免疫をより長期間にわたって高いレベルで維持することが可能なトリインフルエンザウイルス抗原及び経口投与で有効な粘膜アジュバントを含む、免疫材料の提供。【解決手段】オイルワクチンを筋肉内接種した鶏に対して、ELISA抗体価が減少した時点で、遺伝子組換え技術により作成した組み換えHA抗原及び組み換えNP抗原と粘膜アジュバントである合成オリゴCpGを経口投与し、オイルワクチンで付与した免疫応答が弱化しても、組み換えHA抗原及び組み換えNP抗原と合成オリゴCpGを混合して経口投与することで抗体価が再誘導された、免疫材料。【選択図】なし
