An ejecting-clamping-pulling combined seedling fetching device for an automatic transplanter and an operating method therefor. The device comprises: a seedling ejecting mechanism (1), a seedling feeder mechanism (2), a seedling fetching mechanism (3), and a control system. The seedling feeder mechanism (2) is mounted on a stander; the seedling ejecting mechanism (1) is mounted on the seedling feeder mechanism (2) and is located at the rear side of the seedling feeder mechanism (2); the seeding fetching mechanism (3) is mounted at the stander and is located at the front side of the seedling feeder mechanism (2). An upper ejector rod support (1-6) and a lower ejector rod support (1-4) are provided on the seedling ejecting mechanism (1); the seedling ejecting rods on the two supports are disposed in a staggered manner, and the upper ejector rod support (1-6) and the lower ejector rod support (1-4) are configured to alternately eject and loosen seedling trays. The ejecting-clamping-pulling combined seedling fetching device can eject and loose seedling trays, and the completeness of the trays is guaranteed. The ejecting-clamping-pulling seedling fetching method can accurately control seedling fetching and feeding actions; and furthermore, the device is simple in structure, high in reliability and efficiency and low in damage rate of tray bodies.