The present invention relates to a method of determining a metabolic adaptation of a living entity of interest to a first set of environmental conditions and to a second set of environmental conditions comprising (a) determining with a first substrate concentration at least two activities of at least one enzyme comprised in a specimen of said living entity maintained under said first set of environmental conditions and at least two activities of said at least one enzyme comprised in a specimen of said living entity maintained under said second set of environmental conditions, wherein said activities are determined at two non-identical points in time t1 and t2 after starting the determining reaction； (b) determining with a second substrate concentration at least two activities of at least one enzyme comprised in a specimen of said living entity maintained under said first set of environmental conditions and at least two activities of said at least one enzyme comprised in a specimen of said living entity maintained under said second set of environmental conditions, wherein said activities are determined at two non-identical points in time t3 and t4 after starting the determining reaction； wherein said second substrate concentration is at most twofold, preferably is about equal to or lower than, the KM of said enzyme for said substrate； and (c) determining the metabolic adaptation of said living entity based on comparing at least one non-linear activity determined in step (a) and/or (b) to at least one further activity determined in step (a) and/ or (b).The present invention also relates to devices and further methods related thereto； as well as to a redox-fixed HMGB1 derivative polypeptide.La présente invention concerne un procédé de détermination d'une adaptation métabolique d'une entité vivante d'intérêt à un premier ensemble de conditions environnementales et à un second ensemble de conditions environnementales consistant (a) à déterminer avec une première concentr
