A bubble reduction device, chromatography device, bubble reduction method and bubble reduction program capable of reducing bubbles in an eluent. Included are a liquid accommodation portion (12A), a liquid supply apparatus (63), an air layer formation apparatus (72), a first channel (14) and an evacuation portion (76). The liquid accommodation portion (12A) accommodates a liquid that is to elute an analysis component from a specimen adsorbed to an adsorption portion. The liquid supply apparatus (63), by operation of a rod (66) pushing up and pulling down, sucks and discharges the liquid through an aperture portion of a tube portion (65), the aperture portion being oriented upward. The air layer formation apparatus (72) forms an air layer in the tube portion (65). The first channel (14) connects the liquid supply apparatus (63) with the liquid accommodation portion (12A). The evacuation portion (76) is connected to the first channel (14) via a first switching valve (74) and evacuates the air layer through the first channel (14).
