A method for disinfecting and sterilizing an animal tissue material and a corresponding soak solution for an animal tissue. The method includes placing the animal tissue material into an alkaline soak solution containing a metal peroxide and a detergent, and shaking； removing organic components released from microorganisms and animal tissue cells by soaking and washing in a neutral cleaning solution； washing the tissue matrix with a weak acidic cleaning solution； cryopreserving or freeze-drying the tissue matrix in a neutral solution. The soak solution for the animal tissue contains 0.01∼0.2% (w/v) of the metal peroxide and 0.05∼1.0% (w/v) of the detergent. The animal tissue pretreated by the method is advantageous to the preservation and further decellularization treatment of the tissue for manufacture of an intact scaffold material of a tissue matrix.
