A nanocomplex useful for efficient transfection and delivery of biomolecules comprising amphipathic peptide sequence is provided. For example, amphipathic peptides for the delivery of biomolecules to eukaryotic cells are provided. These peptide based vectors can form stable nanocomplex with biomolecules mainly with nucleic acids and can deliver it efficiently to cells. The complexation can be done non-covalently with small as well as large biomolecules.