The present invention provides an oncolytic virus encoding a bispecific molecule specifically recognizing a tumor antigen and a cell surface molecule on an effector cell, such as CD3 on T lymphocytes, wherein the bispecific molecule has low-binding affinity for both tumor antigen and cell surface molecule. The present invention also provides methods and pharmaceutical compositions for cancer treatment, by employing the oncolytic virus described herein. The present invention further provides novel anti-EpCAM antibodies and nucleic acids encoding thereof. Also provided are methods of preparation and kits comprising any of the aforementioned compositions.本發明提供一種編碼雙特異性分子之溶瘤病毒,該雙特異性分子特異性識別腫瘤抗原及效應細胞上之細胞表面分子,諸如T淋巴細胞上之CD3,其中該雙特異性分子對於腫瘤抗原及細胞表面分子二者均具有低結合親和力。本發明亦提供藉由採用本文所描述之溶瘤病毒治療癌症之方法及醫藥組合物。本發明進一步提供新穎抗EpCAM抗體及其編碼核酸。亦提供前述組合物中之任一種之製備方法及包含前述組合物中之任一種的套組。
