A needle groove body, a puncture frame body and a puncture frame, wherein the needle groove body is a hollow cylindrical structure, and comprises a first baffle, a first fixing plate, a limiting plate, and a second fixing plate which are connected in sequence. The needle groove body has multiple specifications and can adapt to puncture needles of different specifications in puncture operations； a u-shaped needle groove can be formed between the first baffle of the needle groove body and a second baffle as well as an inclined mounting plate of the puncture frame body so as to accommodate free movement of a puncture needle in the u-shaped needle groove； and the u-shaped needle groove, which is semi-open, can implement a function of needle-frame separation (separation of the puncture needle from the puncture frame) in puncture operations.
