PROBLEM TO BE SOLVED: To provide a novel DNA aptamer useful for diagnosis, treatment, metastasis prevention or the like of lung cancer.SOLUTION: There are provided a DNA aptamer that has a nucleotide sequence shown by a specific sequence and is specifically bound to a non-small-cell lung cancer cell, a composition containing a DNA aptamer for detecting a lung cancer cell, a kit containing a DNA aptamer for detecting a lung cancer cell, a method for detecting lung cancer using a DNA aptamer, a detection method including: a process of bringing a DNA aptamer into contact with a sample collected from an organism which is selected from the group consisting of a lung cell, lung tissue, blood, serum, plasma, saliva, and sputum and a process of detecting the existence of a lung cancer cell by observing response caused by a bond between the sample and the DNA aptamer, and a pharmaceutical composition containing a DNA aptamer for metastasis prevention or treatment of lung cancer.SELECTED DRAWING: Figure 1COPYRIGHT: (C)2016,JPO&INPIT【課題】肺がんの診断、治療、及び転移予防等に有用な新規DNAアプタマーの提供。【解決手段】特定の配列で示されるヌクレオチド配列を有し、非小細胞肺がん細胞に対して特異的に結合するDNAアプタマー、DNAアプタマーを含む肺がん細胞の検出用組成物、DNAアプタマーを含む肺がん細胞の検出用キット、DNAアプタマーを用いる肺がんの検出方法、DNAアプタマーを肺細胞、肺組織、血液、血清、血漿、唾液、及び喀痰よりなる群から選択される生体から採取された試料と接触させる工程、及び試料とDNAアプタマーとの結合による応答を観測することによって肺がん細胞の存在を検出する工程を含む検出方法、DNAアプタマーを含有する肺がんの転移予防又は治療用医薬組成物。【選択図】図1