A non-invasive system (100) and method for locating blood vessel and analyzing blood of a subject under observation have been disclosed. The system (100) comprises a processor (108), an imaging module in communication with said processor (108) to capture at least a portion of a subject under observation and a display module (112) in communication with said processor to display said portion of the subject under observation (116). In further embodiments said processor (108) is configured to receive data from said imaging module and to construct a surface map of said portion of said section of said surface under observation (116).