The present invention discloses a 99mTc-labeled isocyanide-containing glucose derivative having a structural formula of [99mTc- (CNDG) 6] + and a method for preparing and applying the same. This derivative has 99mTc + as a central nucleus, and a carbon atom of isocyanide in CNDG is coordinated with 99mTc (I) to form a hexacoordinate [99mTc- (CNDG) 6] + complex. The synthesis of the ligand CNDG and the preparation of the freeze-drying kit for CNDG yield [99mTc- (CNDG) 6] + derivatives. The derivatives of the present invention have good stability, are easy to prepare, have relatively high uptake and good retention at tumor sites, have high tumor / non-target organ ratios, Is a new 99mTc-labeled isocyanide-containing glucose derivative that can be used for excellent tumor imaging, and is easily applied widely.
