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STREPTOMYCES MICROFLAVUS STRAINS AND METHODS OF THEIR USE TO CONTROL PLANT DISEASES AND PESTS
专利权人:
Bayer CropScience LP
发明人:
Brian CAMPBELL,Damian CURTIS,Shaohua GUAN,Magalie GUILHABERT-GOYA,Daniel M. JOO,Tara LU,Jonathan S. MARGOLIS,Reed Nathan ROYALTY,Gerardo Bueno SALAZAR,David SESIN,Frisby Davis SMITH,Colleen TAYLOR,Hon
申请号:
US14052094
公开号:
US20140105862A1
申请日:
2013.10.11
申请国别(地区):
US
年份:
2014
代理人:
摘要:
The present invention relates to novel strains of Streptomyces microflavus and methods of their use for controlling diseases or pests of a plant. The invention also relates to a fermentation broth obtained by cultivating a gougerotin producing Streptomyces strain, wherein the fermentation broth contains at least about 1 g/L gougerotin. The invention also relates to a method of producing a fermentation broth of a gougerotin producing Streptomyces strain, wherein the fermentation broth contains at least about 1 g/L gougerotin, the method comprising cultivating the Streptomyces strain in a culture medium containing a digestible carbon source and a digestible nitrogen source under aerobic conditions, wherein the culture medium contains an amino acid at a concentration effective to achieve a gougerotin concentration of at least 1 g/L. The present disclosure also relates to the molecular cloning of a gougerotin biosynthetic gene cluster from Streptomyces microflavus, and identification of individual genes in the gene cluster as well as the proteins encoded thereby. A gougerotin gene cluster comprising 13 open reading frames (ORFs) is located within a genetic locus of Streptomyces microflavus.
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中国工程科技知识中心
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