The present invention relates to novel strains of Streptomyces microflavus and methods of their use for controlling diseases or pests of a plant. The invention also relates to a fermentation broth obtained by cultivating a gougerotin producing Streptomyces strain, wherein the fermentation broth contains at least about 1 g/L gougerotin. The invention also relates to a method of producing a fermentation broth of a gougerotin producing Streptomyces strain, wherein the fermentation broth contains at least about 1 g/L gougerotin, the method comprising cultivating the Streptomyces strain in a culture medium containing a digestible carbon source and a digestible nitrogen source under aerobic conditions, wherein the culture medium contains an amino acid at a concentration effective to achieve a gougerotin concentration of at least 1 g/L. The present disclosure also relates to the molecular cloning of a gougerotin biosynthetic gene cluster from Streptomyces microflavus, and identification of individual genes in the gene cluster as well as the proteins encoded thereby. A gougerotin gene cluster comprising 13 open reading frames (ORFs) is located within a genetic locus of Streptomyces microflavus.本發明關於新穎之細黃鏈黴菌(Streptomyces microflavus)株及使用彼等來控制植物疾病和害蟲之方法。本發明亦關於藉由培養產製谷氏菌素(gougerotin)之鏈黴菌株所獲得的醱酵基質，其中該醱酵基質含有至少約1克/升之谷氏菌素。本發明亦關於製造產製谷氏菌素之鏈黴菌株的醱酵基質之方法，其中該醱酵基質含有至少約1克/升之谷氏菌素，該方法包含在有氧條件下，在含有可消化之碳源及可消化之氮源的培養基中培養鏈黴菌株，其中該培養基含有能有效達成至少1克/升之谷氏菌素濃度的胺基酸濃度。本揭露內容亦關於從細黃鏈黴菌進行谷氏菌素生物合成基因簇之分子選殖，及鑑定該基因簇中之個別基因和由其編碼之蛋白質。包含13個開放閱讀框(ORFs)之谷氏菌素基因簇係位在細黃鏈黴菌之基因位點內。
