The present invention relates to vaccines containing enveloped viruses whose envelopes have been depleted of lipids using methyl β-cyclodextrin (MBCD). Delipidation of enveloped viruses in a two-step process abolishes the infectivity of those viruses, which allows delipidated viruses to be used safely in vaccines. Use of MBCD to deplete lipids such as cholesterol, in contrast to other methods, results in viruses with less than 20% of the cholesterol of an untreated virus but such delipidated viruses retain at least 85% of the protein content of an untreated virus. Delipidation by MBCD also preserves the immunogenicity of the viral proteins. The present invention of using MBCD treatment in a two-step process to delipidate enveloped virus represents a new alternative for generation of inactivated viruses which are incorporated into effective vaccines.本發明係關於含有包膜病毒之疫苗，該等包膜病毒之包膜已使用甲基β-環糊精(MBCD)除去脂質。在兩步過程中對包膜病毒進行去脂質化消除彼等病毒之感染性，此允許去脂質病毒安全地用於疫苗中。與其他方法相比，使用MBCD來去除諸如膽固醇之脂質，產生具有少於20%之未經處理病毒之膽固醇的病毒，但此類去脂質病毒保留至少85%的未經處理病毒之蛋白質含量。藉由MBCD進行去脂質化亦保留該等病毒蛋白質之免疫原性。本發明在兩步過程中使用MBCD處理來對包膜病毒進行去脂質化代表一種產生併入至有效疫苗中之不活化病毒的新替代方案。
