The invention relates to a detection device for detecting a sign of life for an aquatic animal and a detection method thereof. Primarily, the detection device comprises a controller circuit a sensing device electrically connected to the controller circuit. A microprocessor, a signal generating circuit and a data reading circuit are built in the controller circuit, and the sensing device is arranged on an aquatic animal, thus an electrical loop is formed between the aquatic animal and the controller circuit. A signal is output by the signal generating circuit to the sensing device, and the voltage value generated form the body impedance of the aquatic animal corresponding to the output signal of the signal generating circuit is received by the data reading circuit. Furthermore, a method using the device to detect a sign of life of an aquatic animal is provided. Accordingly, it can detect whether or not the aquatic animals are still alive and learn if the aquatic animals are fresh.本發明係有關於一種水產動物生命跡象偵測裝置,主要包括有一控制器電路,並內建有微處理器及分別與其電性連接之訊號產生電路和資料讀取電路;以及,一與控制器電路電性連接之感測裝置,上述感測裝置係設置於水產動物上,使水產動物與控制器電路形成一電性迴路,於訊號產生電路輸出一信號至感測裝置,並於資料讀取電路接收水產動物之體阻抗對訊號產生電路輸出信號所產生之電壓值;本發明還提供一種使用此裝置偵測水產動物生命跡象之方法;藉此,可偵測水產動物是否存活,並得知水產動物新鮮與否。(1)‧‧‧控制器電路(11)‧‧‧微處理器(12)‧‧‧訊號產生電路(13)‧‧‧資料讀取電路(2)‧‧‧感測裝置(21)‧‧‧第一感測電極(22)‧‧‧第二感測電極(3)‧‧‧水產動物