PROBLEM To provide an oil containing a highly unsaturated fatty acid that has a lower content of cholesterol. MEANS FOR SOLVING A method for producing a highly unsaturated fatty acid-containing glyceride is characterized in that the method comprises the steps of: in a method to concentrate a highly unsaturated fatty acid removing the free form of the sterol from a raw material oil that includes highly unsaturated fatty acid-containing glycerides prior to performing a lipase reaction and thereafter concentrating the highly unsaturated fatty acid in the highly unsaturated fatty acid-containing glycerides by using a lipase of low reactivity with respect to the highly unsaturated fatty acid. In the produced highly unsaturated fatty acid-containing glycerides, content of the sterol is 0.3 weight% or less, and content of the highly unsaturated fatty acid is 40 area% or more.
