The primary objective of the present invention is to provide a positioning device for surgical operation, which could allow a surgical tool to be positioned precisely at a specific operating spot or a specific operating angle by providing a three axial free-moving function and a vertical and horizontal free-rotating function. Furthermore, the present invention is able to be electrically connected to and controlled by an automatic control system to achieve a more precise and efficient positioning function.本發明係揭露一種手術定位裝置,其可於手術中提供三維空間之線性位移的操作自由度,以及提供垂直、水平旋轉上之操作自由度,讓一手術工具可隨需求位移至任一特定之手術特定位置、角度上以利手術之進行,且更可藉由自動控制之手段針對三維空間之線性位移以及垂直、水平向之旋轉做高精準度的驅控作動,進而達到高精準度之定位效果。(10)‧‧‧承架(20)‧‧‧橫向線性驅動組件(21)‧‧‧第一軌道件(30)‧‧‧垂直線性驅動組件(31)‧‧‧第二軌道件(32)‧‧‧第二驅動件(33)‧‧‧第二馬達裝置(40)‧‧‧水平旋轉驅動組件(41)‧‧‧第一基座(42)‧‧‧第一旋轉件(43)‧‧‧第三馬達裝置(50)‧‧‧垂直旋轉驅動組件(51)‧‧‧第二基座(52)‧‧‧第二旋轉件(53)‧‧‧第四馬達裝置(60)‧‧‧縱向線性驅動組件(61)‧‧‧第三軌道件(62)‧‧‧第三驅動件(63)‧‧‧第五馬達裝置(70)‧‧‧手術工具端(71)‧‧‧手術工具