Martin G. Martin,James C.Y. Dunn,Matthias Stelzner
申请号:
US14853564
公开号:
US20160068805A1
申请日:
2015.09.14
申请国别(地区):
US
年份:
2016
代理人:
摘要:
A cell culture media composition for support growth of human SI stem cells and epithelium without a feeder layer is presented. The media may also include growth factors including ENR and Y-27632 that support the survival of stem cell spheroid structures. The cell culture media compositions permit rapid growth of human small intestinal (SI) epithelium and stem cells, which leads to specific spheroid cell and enteroid morphology when grown in 3-D culture system.