Kishore Ganesh M.,Motion Michael,Hicks Paula M.,Hansen Jorgen,Houghton-Larsen Jens,Hansen Esben Halkjaer,Mikkelsen Michael Dalgaard,Tavares Sabina,Blom Charlotte
申请号：
NZ70807811
公开号：
NZ708078A
申请日:
2011.06.02
申请国别(地区):
NZ
年份:
2017
代理人:
摘要：
Disclosed is a method for transferring an additional sugar moiety to the C-2’ position of a glucose in a steviol glycoside, comprising contacting the steviol glycoside with a polypeptide capable of beta 1,2 glycosylation of the C2’ of the 13-O-glucose, 19-O-glucose, or both 13-O-glucose and 19-O-glucose of a steviol glycoside, and a UDP-sugar under suitable reaction conditions for the transfer of the additional sugar moiety to the steviol glycoside, wherein the steviol glycoside is steviol-13-O-glucoside, rubusoside, stevioside, or rebaudioside A, and wherein a stevioside, rebaudioside E, rebaudioside D, steviol-1,2 bioside, steviol-1,2-xylobioside, steviol-1,2-rhamnobioside, 1,2-stevioxyloside, an isomer thereof, and/or a steviol glycoside composition thereof is produced upon transfer of the additional sugar moiety.
