A traditional Chinese medicine composition with an anticancer effect, a preparation method therefor, and an application of the traditional Chinese medicine composition in preparing a cancer treatment medicine. The traditional Chinese medicine composition consists of the following raw pharmaceutical ingredients: 20 to 120 parts of rhubarb, 7 to 30 parts of sappan wood, 7 to 30 parts of evodia rutaecarpa, 7 to 30 parts of artemisiae argyi, 90 to 200 parts of motherwort, 3 to 20 parts of turmeric, 15 to 60 parts of white peony roots, and 7 to 30 parts of faeces of flying squirrel by weight. The preparation method comprises the steps: step 1: performing distillation and extraction from turmeric steam, and collecting volatile oil and distillate; step 2: performing extraction on dregs left in the steam extraction in step 1 and on other raw medicinal materials by using an organic solvent soluble with water, and concentrating the extracted solution to obtain a cream; and step 3: mixing the cream obtained in step 2 with the volatile oil and distillate obtained in step 1. Compared with a control medicine, the medicine with a same dosage in the present invention has a higher inhibition ratio on Hela cells than that of the control medicine.La présente invention concerne une composition de médecine chinoise traditionnelle ayant un effet anticancéreux, un procédé de préparation de celle-ci, et une application de la composition de médecine chinoise traditionnelle dans la préparation d'un médicament de traitement anticancéreux. La composition de médecine chinoise traditionnelle est constituée des matières premières pharmaceutiques suivantes : 20 à 120 parties de rhubarbe, 7 à 30 parties de bois de sappan, 7 à 30 parties d'Evodia rutaecarpa, 7 à 30 parties d'Artemisiae argyi, 90 à 200 parties d'agripaume, 3 à 20 parties de curcuma, 15 à 60 parties de racines de pivoine blanche, et 7 à 30 parties de fèces d'écureuil volant. Le procédé de préparation comprend les étapes suivantes : ét