A nucleotide complex for gene silencing is disclosed, which includes: a nanoparticle, and at least one of a first polynucleotide chain and a second polynucleotide chain, cohere in the first polynucleotide chain and the second polynucleotide chain respectively bind to the nanoparticle, and specifically recognize a sequence of target nucleotide. The first polynucleotide has a first end and a relative second end, and the first end partial pair with the second end sequentially to form a hairpin-looped structure and the second polynucleotide chain has a linear structure.本發明係有關於一種用於基因沉默之核酸複合物,包括:一奈米粒;以及一第一多核苷酸鏈及一第二多核苷酸鏈之至少一者,其中該第一多核苷酸鏈及該第二多核苷酸鏈係分別與該奈米粒連接,該第一多核苷酸鏈及該第二多核苷酸鏈係分別專一性辨識一標的核酸之序列,該第一多核苷酸鏈具有一第一端及一相對之第二端,該第一端連接該奈米粒,且該第一端與該第二端的部分序列配對使該多核苷酸鏈呈髮夾式,而該第二多核苷酸鏈係呈直鏈式。1‧‧‧奈米粒21‧‧‧直鏈式多核苷酸鏈22‧‧‧髮夾式多核苷酸鏈221‧‧‧第一端222‧‧‧第二端3,4‧‧‧核酸複合物
