The present invention provides a full human anti-TNF-.alpha. monoclonalantibody, the preparationmethod and use thereof. The antibody in the present invention has an aminoacid sequence ofheavy chain variable region as shown in SEQ ID NO: 6 and an amino acidsequence of lightchain variable region as shown in SEQ ID NO: 8. The antibody in the presentinvention can beused to prepare medicines for the treatment of autoimmune disorders.