A glucose measuring device and apparatus are provided. The device includes a substrate, a cover plate, an electrode assembly, and a reactive unit. The substrate has a first and second surfaces opposite to each other, and a flow channel located at the first surface. The flow channel includes a sampling region having a sample inlet, a measuring region, and a concentrating region therebwtween. The cover plate having a gas outlet is disposed on the first surface and covers the flow channel. The electrode assembly includes a first, a second and a third electrode pairs. The first electrode pair is at a boundary between the sampling and the concentrating regions. The second electrode pair is at a boundary between the concentrating and the measuring regions. The third electrode pair is located in the measuring region. The reactive unit is disposed on the third electrode pair and in the flow channel.
