The invention relates to a use of a pepino (Solanum muricatum Ait) extract for manufacturing composition for decreasing pathogenic bacteria by applying an effective does of the pepino extract to a subject in need, so as to decrease the number of Clostridium perfringens in intestine. Accordingly, the pepino extract can be further utilized in manufacturing health foods for inhibiting growth of pathogenic bacteria.本發明係有關於一種香瓜茄萃取物用於製備減少腸道害菌之組合物之用途,其係施予一有效劑量之香瓜茄(Solanum muricatum Ait)萃取物於一所需個體,以減少腸道內產氣夾膜梭菌(Clostridium perfringens)的數量;藉此,香瓜茄萃取物可進一步運用於作為抑制腸道壞菌生長之保健食品。