A biomolecule extraction device and method for use thereof are disclosed. The biomolecule extraction device has an insertion part, a body part and at least on discharge port. The insertion port is insertable in a body part, and is configured to fix a sample of collected biomass containing biomolecules. The body part is configured to receive a lyses buffer and contact the surface of the fixed biomass with the lyses buffer to extract the biomolecules from the biomass when the insertion part is inserted in the body part. The at least one discharge part is disposed in the insertion part or the body part for discharging biomass lysate containing the extracted biomolecules.