THE ROYAL INSTITUTION FOR THE ADVANCEMENT OF LEARNING/MCGILL UNIVERSITY
发明人:
Brian J. Ward,Kaitlin Grace Frances Winter
申请号：
US16576527
公开号：
US20200093912A1
申请日:
2019.09.19
申请国别(地区):
US
年份:
2020
代理人:
摘要：
An attenuated Salmonella enterica serovar Typhimurium strain (YS1646) is repurposed to produce a vaccine. Plasmid-based candidates expressing either the TcdA or TcdB RBD were screened. Different vaccine routes and schedules were tested to achieve detectable serum and mucosal antibody titers in C57BL/6J mice. When given in a multi-modality schedule over 1 week (day 0 IM+PO, days 2 and 4 PO), several candidates provided 100% protection against lethal challenge. Substantial protection (82%) was achieved with combined PO TcdA/TcdB vaccination alone (d0, 2 and 4). These data demonstrate the potential of the YS1646-based vaccines for C. difficile.
