Richard Tavare,Gregory Mullen,Philip Blower,Rafael Torres Martin de Rosales,Jennifer Williams
申请号：
US13390673
公开号：
US20120251446A1
申请日:
2010.08.18
申请国别(地区):
US
年份:
2012
代理人:
摘要：
Bioconjugates for use in imaging are disclosed include a linker sequence fused to a polypeptide capable of binding a target in a biological system. The linker sequence is designed so that it is capable of being radiolabelled, e.g. with a complex comprising a radionuclide, via a free cysteine residue and the polyhistidine sequence in the label that are both capable of simultaneously binding to a complex comprising the radionuclide. These interactions can improve significantly the rate and efficiency of radiolabelling compared to either protein with the His-tag or the free cysteine alone. Optionally, the free cysteine residue provides a site which can be covalently bonded to a moiety such as a second label, in a site-specific manner.
