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METHOD FOR OBTAINING BACTERIOPHAGE STRAIN, SPECIFIC BACTERIOPHAGE STRAINS AND THEIR APPLICATION
专利权人:
Протеон Фармасьютикалз С.А.
发明人:
ДАСТЫХ Ярослав,ДЗЯДЕК Ярослав,ГУРЕЦКАЯ Эльжбета,РУМИЕВСКАЯ-ГАЛЕВИЧ Анна,ВОЙТАСИК Аркадиуш,ВУЙЦИК Эвелина
申请号:
RU2014111125
公开号:
RU2014111125A
申请日:
2012.08.09
申请国别(地区):
RU
年份:
2015
代理人:
摘要:
1. A method of producing a bacteriophage strain specific for the selected bacterial strain, characterized in that a) a collection of bacteriophage strains is obtained, including a bacteriophage strain specific for the selected bacterial strain, b) the selected bacterial strain is cultured on a sterile culture medium, c) culture samples are applied on a special multi-well measuring tablet, then add a suspension of the test bacteriophage strain at various concentrations and incubate at 37 ° C for at least 4 hours, d) resazurin is added to the culture samples and incubation is continued in the dark at about 37 ° C for at least 3 hours; e) the color or fluorescence of the culture is monitored, as well as the bacteriophage strain contained in the culture that remains blue or does not show significant the increase in fluorescence compared to the control sample is identified as a bacteriophage strain specific for the selected bacterial strain, while a sterile sample that was treated with the same incubation is used as a control sample, f) propagate the identified bacteriophage strain specific for the selected bacterial strain. 2. A method according to claim 1, characterized in that the selected bacterial strain is a strain of S. enterica serovar Enteritidis. 3. The use of bacteriophages in the preparation of a drug for the prevention or control of infections of farm animals, in particular poultry caused by pathogenic strains of bacteria sensitive to these phages, while the resulting product is intended for administration to a vulnerable animal with food or water at intervals of one1. Способ получения штамма бактериофага, специфического к выбранному штамму бактерий, отличающийся тем, чтоa) получают коллекцию штаммов бактериофагов, включающую штамм бактериофага, специфический к выбранному штамму бактерий,b) осуществляют культивирование выбранного штамма бактерий на стерильной культуральной среде,c) образцы культуры наносят на специальный многолуночный измерительный планшет,
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