An extract of Asplenium nidus Linn. for manufacturing a drug or healthy product to treat of prostate diseases is disclosed. The extract of Asplenium nidus Linn. comprises pyropheophorbide a methyl ester (C34H36N4O3), pheophorbide a methyl ester(C35H36N4O5), 1-linleoyl-3-linolenoyl-glycerol (C39H66O5), 1-linleoyl-2,3-dipalmitoyl-rac-glycerol (C53H98O6) and 1,3-dipalmitoyl-sn-glycerol (C35H68O5). The extract of Asplenium nidus Linn. is obtained by using a manufacture method comprising steps of: solvent extracting, using an extract solvent to extract an Asplenium nidus Linn. sample and to obtain an extract, with a w/v ratio between the extract solvent and the Asplenium nidus Linn. sample being 50-60mg/ml; and column chromatography, fractionating the extract with water and ethanol as eluent to obtain several fractions including fraction a to fraction i, and further evaporating and lyophilizing fraction b, c, d, or their combination to obtain an extract of Asplenium nidus Linn.一種台灣山蘇萃取物,係供製備改善攝護腺疾病之藥物或保健食品,包含焦脫鎂葉綠酸-a甲酯、脫鎂葉綠酸-a甲酯、1-亞麻油酸-3-亞麻酸-甘油酯、1-亞麻酸-2,3-雙棕梠酸-甘油酯,以及1,3-雙棕梠酸-甘油酯。該台灣山蘇萃取物藉由一包含下列步驟之方法所製得:一溶劑萃取步驟,係使用一萃取溶劑,對一台灣山蘇樣品進行萃取處理,使該台灣山蘇樣品與該萃取溶劑之重量體積比(w/v,g/ml)為50至60mg/ml,並回收得一萃取物;以及一管柱層析步驟,利用水和乙醇作為流動相,進行該萃取物之層析分離處理,以獲得數個區分物,沖提先後而編號為區段a至區段i,擷選該區段b、c、d或其組合物經減壓濃縮及冷凍乾燥後,即得該台灣山蘇萃取物。